40ml vial contains 40ml liquid culture medium, with space to add up to 5ml of starter culture.
100ml vial contains 100ml liquid culture medium, with space to add up to 10ml starter culture – though 2-5ml should be plenty!
To Use Vial (To Expand a Liquid Culture/Spore Syringe*)
- In aseptic area, clean work surfaces and vial thoroughly with 70% alcohol.
- Pop off the plastic vial cap to expose the rubber injection membrane.
- Affix needle to your Liquid Culture/spore syringe body with a twist.
- Wipe down the rubber injection port with 70% alcohol.
- Insert the needle through the injection port, and inject 2-5ml of LC/spore solution.
- Incubate at the required temperature for your species.
- Shake occasionally to help break up mycelium as it grows.
- Ready to use!
- Withdraw many syringes of LC to inoculate grain spawn, or even more Liquid Culture Vials!
Our Liquid Culture Vials are carefully prepared in a sterile cleanroom lab environment. Liquid culture medium is autoclave sterilised at 121°c. Our lab filtration is rated to ULPA U15 – far exceeding the industry standard HEPA 13/14.
Vials may contain a small amount of sediment. This is primarily caramelised sugars; a natural result of the autoclave process. This sediment is not detrimental in any way to the mycelium – it is in fact a source of nutrition. The liquid culture medium may become clearer or lighter in colour as the mycelium consumes the nutrients in the liquid – this is perfectly normal.
Please note: * It is advisable to inoculate the Expansion Vial with a clean liquid culture. Multi-Spore Syringes are inherently non-sterile and are more likely to contaminate a liquid culture.